利用组织培养技术筛选辣椒抗疫病变异体

Stydy on the selection of cell mutant lines resistant to Phytophthora capsici in pepper

选用“2001”,“2056”,“2031”3个辣椒品种作为试材,以其子叶作为外植体诱导愈伤组织,以辣椒疫病病原菌培养滤液制取粗毒素作为筛选剂,筛选辣椒抗疫病细胞变异系。结果表明,辣椒疫病病原菌粗毒素对辣椒子叶愈伤组织的诱导、生长及不定芽的分化具有显著的抑制作用,抑制作用随着粗毒素浓度的增加而增加,且不同辣椒品种愈伤组织对毒素的忍耐力不同。

In this study we selected some cell mutant lines by using the cotyledons of three pepper cultivars ＂2001＂,＂2056＂,＂2031＂ as explants induced the callus and the filtrate of Phytophthora capsici （crude toxin） as the selective agent. The experimental results showed that the crude toxin of Phytophthora capsici inhibited the inducement,the growth of callus,and the differentiation frequency and the growth of buds. The inhibition increased with the increase of concentration of crude toxin. The experiment also found that different cultivars had different resistance to Phytophthora capsici.