甜菜苗茎端和薄细胞层的离体培养

DIRCT ORGANOGENESIS FROM SHOOT APICES AND THIN CELL LAYER EXPLANTS IN SUGAR BEET CULTURE IN VITRO

在添加营养液的蛭石上培育的甜菜种子苗,用1/2MS+0.5mg^(L-1)BAP或1/2B_5+0.5mgL^(-1)BAP培养基培养幼苗先端部分,切口处7—10天形成愈伤组织占80%,形成小植株占70%。甜菜叶和叶柄的薄细胞层离体培养,用1/2Ms+0.5mgL^(-1)BAP+0.5mgL^(-1)NAA培养基,愈组率高达80%。

Sugar beet seeds were immersed in 100% alcohol and sterilized with 5% (V/V) 'Teepol' detergent and 15% (V/V) 'Domestos' bleach, and then washed in sterile distilled water. Sterilized seeds were cultured in moist sterile vermiculite, to which appropriate amount of nutrient elements had been added. Seedlings from the seeds grew vigorously and evenly. By culturing the seedling apices on 1/2 Ms or 1/2 B_5 medium, both of which contained 0.5 mg L^(-1) BAP, calluses were formed at the cut ends within 7—10 days at a rate of 75%. Of them 65% produced plantlets. The best medium for the culture of leaf and petiole sub-tissues of sugar beet was found to be 1/2 MS medium containing 0.5 mg L^(-1) BAP and 0.5 mg L^(-1) MAA. The rate of callus production was about 50—80%.