文蛤多糖的提取及含量测定

Extraction and determination of polysaccharides from Meretrix meretrix Linnaeus

目的确定文蛤多糖提取的最佳工艺，以苯酚-硫酸显色，分光光度法测定文蛤多糖含量。方法采用超声波提取，酸水解法进行处理；利用苯酚-硫酸显色。标准曲线在490nm处分光光度法测定。结果超声提取30min，提取2次，料水比1：20为最佳工艺。曲线在4．848～24．242mg·L^-1范围内呈良好的线性关系，回归方程：C=28．69A＋0．229（r=0．9992）;平均回收率为100．67％，RSD为2．83％（n=6）。结论本实验文蛤多糖的提取厦含量测定操作简便，结果准确，重现性好，可用于文蛤多糖的提取与含量测定。

Objective To study the optimum extration process of polysaccharides from Meretrix meretrix Linnaeus. A spectrophotometry method for content determination of polysaccharides from Meretrix meretrix Linnaeus was established with Phenol-sulfuric acid coloration. Methods The samples were extracted by ultrasonic wave, dissolved in 50mL, 4mol·L^-1 HCl and heated to hydrolyze by acid. The contents of polysaccharides from Meretrix meretrix Linnaeus were determined by spectrophotometry at 490nm. Results Extracted for thirty minutes by ultrasonic wave, two times, and acidic hydrolysis were the optimum technology. The calibration curve was linear over the range of 4. 848-24. 242mg·L^-1. The regression equation was C=28.69A＋0. 229（r=0. 9992）. The average recovery rate was 100.67% and RSD was 2.83%（n=6）. Conclusion The method was proved to be simple, accurate and reliable, and could be used to extract and determine the polysaccharides from Meretrix meretrix Linnaeus.