筛选妊娠糖尿病巨大儿大鼠模型的新方法

Screening of Gestational Diabetes Mellitus Models with Macrosomia in Rats: A New Approach

目的建立妊娠糖尿病巨大儿大鼠模型。方法SD雌性大鼠腹腔注射链脲佐菌素(streptozotocin,STZ)25 mg/kg,之后与同系雄性大鼠合笼见栓者定为孕0 d。孕6 d测定血糖高出孕前20%者为模型组(M组),与正常对照组(C组)继续妊娠至孕21 d行剖腹产术,分离胎鼠。结果M组胎鼠体重、身长、尾长均显著大于C组(均P<0.001)、胎鼠血糖高于C组(P<0.001)。结论腹腔注射25 mg/kg STZ后挑选孕6 d血糖高于孕前20%者可复制妊娠糖尿病巨大儿大鼠模型。

Objective To establish gestational diabetes mellitus model with macmsomia in rats Methods 7 days after being injected STZ 25mg/kg intraperitoneally, Female SD rats were mated with the male. On the day 6 of conception, plasma glucose （PG） levels were determined and those whose PG level increased by 20% than that before pregnancy were selected as the Model （M） group. All rats were e-sectioned on the day 21 of pregnancy to observe the fetuses. Results Body weights, body lengths, tail lengths of the offspring in M group were signifleandy heavier or longer than those in C group （ P 〈 0.001 ）. Fetal plasma glucose concentrations in M group were higher than those in C group and the statistic difference was noted （ P 〈 0.001 ）. Conclusion 7 days after being injected STZ 25 mg/kg intmperitoneally, GDM rats with 20% increase of plasma glucose levels on the day 6 of conception than before pregnancy can produce macmsomia.