新药长毒试验动物血液生化测定规范化研究系列之三——血样存放时间对生化值的影响

Effects of Preservation Period of Blood Samples on Biochemical Measurement

目的探讨不同存放时间对所测定血液样本生化值的影响。方法采用全自动生化仪对存放不同时间动物血液中丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、总蛋白(TP)、白蛋白(ALB)、总胆红素(TBil)、碱性磷酸酶(ALP)、尿素(Urea)、肌酐(Cre)、葡萄糖(Glu)、总胆固醇(CHO)等生化值进行测定。结果相同温度存放不同时间,血清生化值出现不同程度的变化。其中,存放时间对TP、ALB及CHO影响最为明显。结论建议在长毒试验动物血液生化测定中,对制备后的血液样本,在一定温度下的存放时间应进行规范与统一。

Objective To observe the effects of preservation period of blood samples on biochemical measurement. Methods By using automatic biochemical analyzer, the biochemical values of blood samples after different preservation periods were measured and analyzed. Results The biochemical values of blood samples after different preservation periods differed significantly. The values of TP, ALB and CHO were more sensitive to preservation periods. Conclusion It is important to unify the nreservation neriod of blood samnles during chronic toxicity testing studies.