摘要
目的:研究胰岛素和胰岛素样生长因子-Ⅰ(IGF-Ⅰ)在高浓度葡萄糖条件下对新生大鼠颅骨成骨细胞葡萄糖载体-1(GLUT1)表达的影响,探讨胰岛素和IGF-Ⅰ对糖尿病性骨质疏松和骨质减少治疗的作用机制。方法:采用组织块法分离培养新生SD仔鼠颅骨成骨细胞,分别给予不同葡萄糖浓度的培养液,即正常浓度葡萄糖(5.5mmol/L)和高浓度葡萄糖(25.5mmol/L)。在高糖条件下分别加入胰岛素(10-6mmol/L)和IGF-Ⅰ(10-7mmol/L)。RT-PCR检测GLUT1mRNA的表达,免疫荧光和WesternBlot检测GLUT1蛋白的表达。结果:与正常浓度葡萄糖组相比,高浓度葡萄糖使成骨细胞GLUT1mRNA和蛋白的表达分别增加51%和35%。胰岛素和IGF-Ⅰ可以下调高糖条件下增加的GLUT1mRNA和蛋白表达,其表达水平与正常浓度葡萄糖条件下的表达水平相似。结论:胰岛素和IGF-Ⅰ纠正了高浓度葡萄糖引起的成骨细胞GLUT1表达的改变可能是治疗糖尿病性骨质疏松和骨质减少的机制之一。
Objective To investigate the effects of insulin and insulin-like growth factor Ⅰ (IGF-Ⅰ) on the expression of glucose transporter 1 (GLUT1) of osteoblasts in high glucose concentration and to explore the therapeutic mechanism of insulin and IGF-Ⅰ for diabetic osteoporosis and osteopenia. Methods Osteoblasts from new born Sprague-Dawley rats were isolated and cultured in media containing either normal (5.5 mmol/L) or high glucose concentration (25.5 mmol/L), respectively administering insulin (10^-6mmol/L) and IGF-Ⅰ (10^-7mmol/L). The level of GLUT1 mRNA was determined by semiquantitativc reverse transcription-PCR. The GLUT1 protein was detected by immunofluorescence and Western Blot. Results The gray scale showed the levels of GLUT1 mRNA and protein in the high glucose group increased 51% and 35% respectively, compared with the levels of their counterparts in the normal glucose group. However, IGF-Ⅰ and insulin could lower the levels of GLUT1 mRNA and protein in the high glucose group. Conclusions The present study showed that insulin and IGF-Ⅰ rectified the change of the expression of GLUT1 mRNA and protein caused by increased level of glucose, which may be involved in the therapeutic mechanism of diabetic osteoporosis and osteopenia.
出处
《实用医学杂志》
CAS
2006年第4期375-377,共3页
The Journal of Practical Medicine