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NASBA和IS-NASBA的临床应用及研究进展 被引量：4

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摘要 核酸序列依赖扩增(NASBA)是一种特异性等温扩增RNA的技术,是基于一个等温扩增酶系统,包括AMV逆转录酶(AMVRT)、RNaseH和T7RNA聚合酶。以RNA为模板,在AMV逆转录酶和引物Ⅰ的作用下合成cDNA,引物Ⅰ的5′端带有T7启动子序列,然后引物Ⅱ与cDNA链退火合成cDNA链的互补链,进而形成了含完整T7启动子序列的双链DNA。T7RNA聚合酶以此双链DNA为模扳,合成大量拷贝的RNA片段。原位NASBA(insituNASBA,ISNASBA)是结合原位杂交技术而发展起来的,主要用于分子病理学、分子形态学领域的杂交技术。
作者 秦维超 周新 张有顺
机构地区 湖北十堰郧阳医学院附属人民医院检验科
出处 《国际检验医学杂志》 CAS 2006年第2期158-160,163,共4页 International Journal of Laboratory Medicine
关键词 基因扩增 RNA 核酸类
分类号 R450 [医药卫生—治疗学]
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参考文献17

  • 1Cook N.The use of NASBA for the detection of microbial pathogens in food and environmental samples.J Microbiol Methods,2003,53(2):165-174.
  • 2Rodriguez-Lazaro D,D'Agostino M,Pla M,Cook N.Construction strategy for an internal amplification control for real-time diagnostic assays using nucleic acid sequence-based amplification:development and clinical application.J Clin Microbiol,2004,42 (12):5832-5836.
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同被引文献41

  • 1陈金顶,索青利,廖明,辛朝安.沙门氏菌的invA基因序列分析与分子检测[J].中国人兽共患病杂志,2004,20(10):868-871. 被引量：58
  • 2单松华,刘乐庭,陈家华,吴仲梁.NASBA快速检测禽流感H5亚型病毒[J].中国病毒学,2005,20(3):288-292. 被引量：18
  • 3寇晓霞,吴清平,范宏英.轮状病毒NASBA检测研究[J].微生物学报,2006,46(2):328-330. 被引量：14
  • 4马保华,李贺,吕平,刘计亮,鄢志强.依赖核酸序列的扩增技术在动物病原微生物检测中的应用[J].动物医学进展,2007,28(4):110-112. 被引量：5
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  • 6Souza D H, Jaykus L A. Nucleic acid sequence based amplifi- cation for the rapid and sensitive detection of Salmonella en- terica from foods[J]. App Microbiol,2003, 95(6) :1343-1350.
  • 7Costa A,Lamb D,Garland S M,et al. Evaluation of light cycler as a platform for nucleic acid sequence-based amplification (NASBA) in real-time detection of enteroviruses[J]. Curr Mi- crobiol,2008,56 : 80-83.
  • 8Weusten J J A M, Carpay W M, Tom A M, et al. Principles of quantitation of viral loads using nucleic acid sequence-based amplification in combination with homogenous detection using molecular beacons[J]. Nucleic Acid Res, 2002, 30, e26.
  • 9Lau L T, ScottM R, Donald P K . Detection of foot and mouth disease virus by nucleic acid sequence-based amplifica- tion(NASBA)[J].Vet Microbiol, 2008, 126(1-3) : 101-110.
  • 10Uyttendaele M, Sehukkink R, Gemen B, et al. Development of NASBA, a nucleic acid amplification system, or identifica- tion of Listeria monocytogenes and comparison to ELISA and a modified FDA method[J].Food Microbiol, 1995,27 (1): 77- 89.

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国际检验医学杂志
2006年 第2期

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