摘要
目的研究板蓝根高极性部位(Ftp)与板蓝根F022部位协同抗内毒素作用。方法ELISA法检测内毒素刺激鼠腹腔巨噬细胞分泌肿瘤坏死因子α(TNF-α)、白介素6(IL-6)水平,内毒素致BCG增敏小鼠死亡实验。结果Ftp联合F022处理巨噬细胞,能显著抑制TNF-α和IL-6水平,效果优于单用F022组;Ftp联用F022显著延长BCG增敏小鼠受内毒素攻击后的存活时间。结论Ftp本身抗内毒素活性较弱,但与F022部位合用,可进一步增强F022部位的抗内毒素作用。
Objective To study the anti - endotoxin synergistic effect of top - polar fraction （ Ftp ） and F022 from Radix Isatidis. Methods The production of TNF -α and IL - 6 of murine peritoneal macrophages stimulated by LPS was measured by ELISA. The lethality of BCG -primed mice was induced by LPS. Results Ftp and Fo22was added simultaneously to macrophages culture lh before addition of LPS, Production was remarkably inhibited. F,pand F022 remarkably protected BCG - primed mice from LPS induced lethality. Conclusion These results suggest that Ftp can develop the anti - endotoxin activity of F022 from Radix Isatidis.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2006年第2期156-157,共2页
Lishizhen Medicine and Materia Medica Research
基金
国家自然科学基金项目(No.39870872)
