摘要
目的 建立降脂减肥颗粒中葛根素的含量测定方法。方法 采用Shim-pack(岛津)ODSC柱(150mm×4.6mm,5μm),流动相为甲醇-0.1%磷酸(25.3:74.7),流速为0.9mg/ml,检测波长为250nm。结果 葛根素在0.1140~0.5700℃范围内线性关系良好,r=0.9996,平均回收率为98.7%,RSD为0.67%。结论 此测定方法准确,重现性好,可作为制剂的质量控制方法。
Objective To establish the HPLC method for the determination of puerarin in Jiangzhijianfei granule. Methods The chromatographic conditions included:The column is Shim-pack ODS C18 column (150 mm × 4.6 mm, 5μm), the mobile phase is methol - water H3PO4 ,the detection wavelength is 250 nm and the column temperature is room temperature. Results The linear range of puerarin was 0.114 0~0. 570 0μg, r = 0.999 6. The average recovery of puerarin was 98.7% with RSD 0. 67%. Conclusion The mothod is accurate and can be used as a determination of puerarin in Jiangzhijianfei granule.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2006年第2期205-206,共2页
Lishizhen Medicine and Materia Medica Research