天花粉蛋白抗人免疫缺陷病毒1型研究

Effect of trichosanthin on suppressing human immunodeficiency virus 1 activity

目的检测用基因工程方法制备的天花粉蛋白抗人免疫缺陷病毒1型的活性。方法将人免疫缺陷病毒1型在细胞系中培养,加入天花粉蛋白后,观察细胞病变情况,检测P24抗原及逆转录酶的活性。结果加入天花粉蛋白的细胞始终未见细胞病变,P24抗原及逆转录酶检测均为阴性;阴性对照组细胞在第7 d以后均出现人免疫缺陷病毒1型感染典型细胞病变,P24抗原及逆转录酶检测均为阳性;阳性对照组始终未见HIV-1感染典型细胞病变,逆转录酶的活性检测和P24抗原检测结果均为阴性。结论天花粉蛋白在培养细胞系中可以有效地抑制人免疫缺陷病毒1型。

Aim To detect the anti-human immunodeficiency virus activity of trichosanthin expressed by the means of bioteehnology. Methods Trichosanthin were put into the cells infected by HIV-1 and the activity of HIV P24 antigen and RT activity were detected. Results It was found that the cells added into trichosanthin showed RT activity negative, P24 Ag negative and no representative cell pathomorphology change while the cells added into distilled water showed RT activity positive, P24 Ag positive and representative cell pathomorphology changed, and the cells added into zidovudine showed RT activity negative, P24 Ag negative and no representative cell pathomorphology change. Conclusion Trichosanthin can inhibit human immunodeficiency virus 1 in vitro.