醋酸铅对小鼠受精能力及受精卵发育毒性研究

The toxicity of the lead acetate on the fertilization ability of sperm and the development of early embryo in mice

目的研究醋酸铅对公鼠精子受精能力及受精卵发育的毒性，为进一步研究提供依据。方法采用腹腔注射染毒法将体重为（18±2）g的性成熟期公鼠随机分为高剂量（1／10 LD50）、中剂量（1／50 LD50）、低剂量（1／250 LD50）染毒组和对照组。染毒组公鼠于试验的0，3，6，9d和12d分别腹腔注射1．2，0．24，0．048mg／mL的Pb（CH3COO）2·3H2O溶液，注射量0．01mL／g，对照组注射相应体积的灭菌蒸馏水。染毒30d后用各组公鼠与未染毒超排母鼠配种。结果回收胚胎中受精并卵裂的胚胎比例随染毒剂量的增加而显著下降，未受精卵比例相应上升。在受精卵地进一步发育中，随着染毒剂量的增加，退化胚比例显著上升，发育到囊胚-孵化胚的比率则显著降低。结论醋酸铅能明显影响小鼠精子的受精能力，并影响受精卵的进一步发育。

Aim To study the toxicity of the lead acetate on the fertilization ability of sperm and the development of early embryo in mice. Methods The puberty male mice in the body weight（ 18±2） g were divided into high dose （1/10 LD50）, moderate dose（1/50 LD50）, low dose （1/250 LD50）, and solvent control group, randomly. The 1.20, 0.24, 0. 048 mg/mL lead acetate [ Pb（ CH3 COO） · 3H2O]solution and distilled water were injected into abdominal cavity （0.01 mL/g） of the mice in poisoned groups and in control group, respectively, with total 5 times. After 30 days, the male mice were used to mate with untreated female mice superovulated. Results The results showed that the fertilizing rate and cleavage rate were decreased significantly, and the ratio of infertilized oocytes were increased with the increasing lead acetate dosages. In the further development process of 2-cell embryo, there still occurred degeneration, so that the development rate of the blastocysts and hatch blastocysts were decreased significantly. Conclusion The lead acetate could remarkably affect the fertilization ability of sperm and the development ability of embryo.