天螺霜对免疫功能低下小鼠模型特异性免疫功能的影响

Effects of Polysaccharide from B.Jiangsiensis to the immune deficit mouse's specific immunological function

目的 研究天螺霜作为免疫增强剂对免疫功能低下模型小鼠特异性免疫功能的影响及其剂量效果关系。方法 采用皮下注射环磷酰胺制造小鼠免疫功能低下模型，观察并比较不同剂量天螺霜（400mg／kg、200mg／kg、50mg／kg）与环磷酰胺配合使用对以鸡红细胞为抗原的溶血素生成和二硝基氯苯所致小鼠迟发型超敏反应的影响。结果 模型组小鼠溶血素、溶血空斑形成和迟发型超敏反应均明显下降．而阳性药物对照组和高、中剂量天螺霜组该免疫功能均明显增强（P〈0．05或P〈0．01），且其作用强度呈现剂量依赖性，高剂量组作用略强于阳性药。结论 天螺霜可明显增强免疫功能低下模型小鼠特异性免疫功能．作用呈剂量依赖性。最高剂量推荐200～400mg／kg。

Objective 20 investigate the enhanced effect of polysaccharide from B.jiangsiensis（PBJ） on the immune hypofunction mouse models and its dose-effect relationship. Methods The immune hypofunction mouse models were made by hypodermic injection with cyclophosphamide（Cy） in mice. PBJ＇s effect on hemolysin content in mouse body induced by chicken erythrocytes and the delayed-type hypersensitivity （DTH） induced by dinitrochlorobenzene in mice were observed and contrasted in the different mouse groups treated with Cy in vivo by hypodermic injection together with PBJ at doses of 400mg/kg,200mg/kg and 50mg/kg, respectively. Results The test showed that the index of hemolysin, hemolytic plaque formation and delayed hypersensitivity reaction in model group markedly decreased while the indexes of hemolysin test in the control group, high and middle dose groups in creased（P 〈 0.05 or P 〈 0.01 ）. The effect of PBJ was close-dependent on the specific immune. Conclusions PBJ showes markedly to increase the specific immune function and to be dose dependent in the effect. The highest dosage recommended ranges from 200 to 400mg/kg.