序贯法和单纯进补法对慢性运动应激大鼠脑组织和胸腺组织糖皮质激素受体的影响

Effects of Order Supplement and Simply SupplementMethod on Glucocorticoid Receptor in BrainCytosol and Glucocorticoid Receptor in Thymusof Rat during Chronic Exercise Stress

探讨慢性运动应激引起免疫抑制的发生机理和中药不同的调理方式对慢性应激下血清皮质酮、脑组织和胸腺组织糖皮质激素受体的影响。研究方法SD雄性大鼠40只,随机平均分为4组,分别为对照组(C)、训练组(T)、服药1组(M1,先补脾,后补肾法,即序贯法)和服药2组(M2,补肾法),采用放免法观察8周递增负荷游泳和中药不同调理方式对不同组别皮质酮和糖皮质激素受体的影响。研究发现,训练组皮质酮显著性高于对照组(P<0.05);而M1组和M2组的皮质酮显著低于训练组(P<0.05,P<0.05);与对照组相比较,训练组脑组织糖皮质激素受体含量显著性低于对照组(P<0.01);M1组和M2组均高于训练组,但无显著性差异(P>0.05;P>0.05);与对照组相比较,训练组胸腺糖皮质激素受体作用位点,显著低于对照组(P<0.01);M1组与训练组相比,显著高于训练组(P<0.01);M2组高于训练组,但没有显著性差异(P>0.05),而M1组高于M2组(P<0.01)。研究结果提示,长期大运动量递增负荷训练可使血清皮质酮水平升高,脑组织GR含量下降,减弱了对糖皮质激素的敏感性,使HPA轴的反馈作用降低。长期大运动量递增负荷训练,可导致胸腺细胞上GR的结合位点明显降低,胸腺细胞上GR水平降低,直接影响免疫细胞的成熟,而降低机体的免疫能力,可能与长时间大强度运动训练会造成免疫抑制,易患呼吸道感染有关。采用序贯法可提高胸腺细胞上GR数目有利于T细胞的成熟和分化,减少糖皮质激素对淋巴细胞的抑制作用,使机体的免疫功能得到改善和提高。

In order to discuss the mechanism of immunosuppression caused by chronic exercise stress and effects of Chinese traditional medicine in the different acting style on serum corticosterone and glucocortlcoid receptor in brain cytosol and glucocorticold receptor in thymus. 40 male SI） rats are randomly divided into four groups;control group （C）, training group （T）,group 1 with medicine （M1） and group 2 with medicine （M2）. Radioimmunoassay is used to observe the effect of 8 weeks swimming training with incremental load and Chinese traditional medicine in different aetlng style on serum corticosterone and glucocortieold receptor in brain cytosol and in the thymus. The result shows that the cortieosterone in training group is significant higher than that of control group,while the corticosterone in M1 and M2 group is significant lower than that of training group. Comparing with control group, the content of glueocorticoid receptor in brain eytosol of training group is significant lower than that of control group, while the M1 and M2 group is higher than training group. There is no significant difference among them. It suggests that long-term incremental training load can increase the level of serum eorticosterone,while the content of GR in brain cytosol decreases. Long-term incremental training load also can lead to the decrease of GR in the thymus,which cut down body immunization capability because the decline of GR level in thymus directly affects immune cell mature, Order supplement can raise the amount of GR in the thymus cell and favor to T cell mature and dlfferentiation,which improve the body immunological function.