球孢白僵菌对竹梢凸唇斑蚜的毒力

Virulence of Beauveria bassiana against Takecallis taiwanus(Takahashi)

测试来自不同虫源的5个球孢白僵菌菌株对竹梢凸唇斑蚜的毒力。结果表明:5个菌株对竹梢凸唇斑蚜都表现出一定的致病力。根据时间-剂量-死亡率模型,考查不同菌株的剂量效应和时间效应的指标值lg(LC50)和LT50,菌株B12,Bxs,REBb01,Zhe-B和F-263在接种后第9天的lg(LC50)值分别为4·0549,4·4620,4·6261,4·9290和5·1580;在1×106个孢子·mL-1浓度下LT50分别为3·63,3·70,4·31,4·34和5·20d。根据该蚜虫累积死亡率随时间和剂量变化的三维曲面图综合评价,5个菌株的毒力从强到弱依次为B12,Bxs,REBb01,Zhe-B和F-263。

Virulence bioassays of the 5 isolates of Beauveria bassiana from different species of insects against Takecallis taiwanus （Takahashi） were carried out. The result showed that all 5 isolates tested were capable of infecting T. taiwanus （Takahashi）. The time-dose-mortality model （namely complementary log-log model） was used for the analysis of the data obtained from the bioassay. Time-dependent lg（LTso） and dose-dependent LCso were estimated respectively. For B12, Bxs, REBb01, Zhe-B and F-263, the lg（LCs0） by the 9^th day after inoculation were estimated to be 4.054 9, 4.462 0, 4.626 1, 4. 929 0 and 5. 158 0 conidia·mL^-1 respectively, and the LTso at an inoculate dose of 1 × 10^6 conidia·mL^-1 were estimated to be 3,63, 3.70, 4.31, 4.34 and 5.20 days respectively. Based on three-dimension figures of cumulative mortality of T. taiwanus（Takahashi） with the changes of time and dose, virulence of each isolate under different time and dose were evaluated integrally. The virulence of 5 isolates in a descending order was B12, Bxs, REBb01, and followed by Zhe-B and F-263.