光敏化BPD-MA后膀胱癌细胞凋亡的研究

Apoptosis of human bladder cancer BIU-87 cells after photodynamic therapy with laser-activated BPD-MA determined with TUNEL assays

为了研究光动力抑癌作用机制,采用流式细胞仪和TUNEL法检测激光光敏化BPD-MA后人膀胱癌细胞株B IU-87细胞凋亡的发生状况。激光光敏化BPD-MA后人膀胱癌细胞株B IU-87组细胞凋亡发生率明显增高,许多细胞核呈棕黄色,高倍镜下可见细胞核内有大量棕褐色颗粒,与阳性对照中凋亡细胞核的特征相符合,而对照组此种细胞少见。TUNEL阴性对照中细胞核均呈蓝色。结果表明,激光激活BPD-MA光动力作用明显诱导人膀胱癌细胞株B IU-87细胞发生凋亡,这可能是其抑癌作用机制之一。

In order to study the mechanism of photodynamie therapy on malignant tumors, photosensitization of BPD-MA is activated by laser with red light（632.8nm） delivered at 10mW/cm^2 to give a total dose of 2.4J/cm^2. Apoptoais of human bladder cancer BIU-87 ceils is determined with flow cytometry with PI staining and terminal deoxyuridine nick end labeling （TUNEL） assays. Results show that photodynamic therapy with BPD-MA significantly increases the rate of apoptusis in BIU-87 cells. A lot of the positive cells in which their nucleus is brown-yellow fine particle shape or overflowing-dispersed are observed after treatment of cells with photodynamic therapy with BPD-MA. However,laser irradiation alone, BPD-MA alone and sham radiation without BPD- MA can not affect apoptosis of human bladder cancer BIU-87 cells. A conclusion can be made that treatment of cells with photodynamie therapy obviously induces human bladder cancer BIU-87 cells to apoptosis, which may be one of the important mechanism of photodynamic therapy on malignant tumors.