植物激素和活性炭对新疆雪莲组培苗生根的影响

Effect of the Plant Hormones and Active Carbon on Rooting of in Vitro Shoot of Saussurea involucrata Kar. et Kir.

研究了不同植物激素和活性炭对新疆雪莲组培苗生根的影响,测定了根长、根粗、根数和生根率4个生根指标,结果表明:添加生长素IAA、IBA和NAA均可以不同程度地促进生根,但0.2mg/L的IAA效果最佳,根长最长达5.5mm,根粗最小0.96mm,根数最多达9.75条,生根率达100%;添加活性炭可以提高生根质量,最适添加量为1mg/mL。此时,根长最长达25.51mm,根粗最小为0.6mm,根数最多达13条,生根效果最佳。

Effect of the plant hormones and active carbon on rooting of in vitro shoots of Saussurea involucrata Kar. et Kir. was studied. Four rooting indexes, including root length, root thick, rooting number and rooting induction rate, were measured. It was concluded that adding the auxins, such as IAA, IBA and NAA, could enhance the induction of the roots, and 0.2mg/L IAA could produce the best result, the length of root is longest （5.5mm）, the root thick is the thinnest （0.96mm）, rooting number is much more than others, the rooting induction rate is 100%. Adding active carbon could improve the quality of the rooting. The optimal concentration was 1mg/mL, the length of root is 25.51mm, the root thick is 0.6mm, and rooting number is 13.