卷丹百合鳞片及珠芽组织培养研究

The Bulb Scale and Bulblet Culture in vitro of Lilium lancifolium Thunb.

以卷丹百合(LiliumlancifoliumThunb.)的鳞片及珠芽作为外植体,比较各种消毒时间和接种方法的效果和不同激素浓度对鳞片及珠芽的诱导增殖效果。结果表明,鳞片消毒以70%酒精20s再用0.1%HgCl212min效果最佳,珠芽消毒以70%酒精20s再用0.1%HgCl210min效果最佳;百合鳞片的分化能力外层好于中层,中层好于内层;近轴面向上的鳞片诱导效果好于远轴面向上。卷丹百合鳞片及珠芽的最佳诱导培养基为MS+6-BA1.5mg/L+NAA0.2mg/L,诱导出生长势较强、数量较多的不定芽。最适合的增殖培养基为MS+6-BA1.0mg/L+NAA0.2mg/L,增殖系数达8.0,组培苗长势良好。

The bulb scales and bulblets culture in vitro of Lilium lancifolium Thunb were studied. The sterilization time, inoculation methods and the effect of plant hormones on inducement and proliferation were compared. The results showed 70% alcohol with 20s and 0.1% Hgcl2with 12min was the best for the bulb scales sterilization and 70% alcohol with 20s, 0.1% HgCl2 with 10min for bulblets. The regeneration ability of external scales was better than the middle ones, which was better than the internal ones. MS＋6- BA1.5mg/L ＋NAA0.2mg/L could induce more and stronger shoots. The best proliferation medium was MS＋ 6-BA1.0mg/L＋NAA0.2mg/L. The multiplication coefficient was 8.0 with strong plantlets.