摘要
采用经典CTAB法、简化CTAB法、氯化苄法和快速提取法对绿色木霉总DNA进行制备。利用依据绿色木霉CBH2基因设计合成的一对引物,以上述方法制备的DNA为模板进行PCR反应,证明简化CTAB法是绿色木霉PCR模板DNA制备的最佳方法。
The total DNA of Trichoderma viride was extracted with the classical CTAB method, simplified CTAB method, benzyl chloride method and a rapid DNA extraction method. A pair of primers were designed and synthesized according to the sequence of CBH2, the CBH2 gene fragment was amplified with PCR, using the DNA extracted with the methods above as template. The result showed that the simplified CTAB method was the best method for the extraction of PCR template of Trichoderma viride.
出处
《纤维素科学与技术》
CAS
CSCD
2006年第1期27-30,共4页
Journal of Cellulose Science and Technology
基金
河南省高校青年骨干教师资助项目
关键词
绿色木霉
PCR模板
制备
Trichoderma viride
PCR template
extraction
