草原红牛及其杂种牛群体遗传变异与肉用性能的微卫星标记研究

Study on the Population Genetic Variation and Microsatellite Marker of Beef Performance of Grassland Red Cattle and Its Hybrid

选用草原红牛、草原红牛与利木赞杂交后代作为试验牛群体,经过基因组DNA的提取、微卫星引物的PCR扩增、扩增产物的电泳分型、各座位等位基因分析以及基因频率、多态信息含量(PIC)和杂合度(H)的计算等步骤,从分子水平上分析了草原红牛及其杂交牛群体的遗传变异。在此基础上,以体重、体尺作为衡量牛生长发育的指标,以肉牛线性体型评分方法中的肌肉度线性评分性状和屠宰肉用性状作为衡量牛肉用性能的指标,运用SPSS软件分析了21个性状与8个微卫星标记的关系,发现了6个微卫星标记对试验牛群体某些生长发育性状和肉用性状存在正面或负面影响。

A group of individuals which included Grassland Red cattle（GLRC） and its improved hybrid by Limousine（LM） was chosen as test population. Its population genetic variation was analysised in the level of molecule by the process of genome DNA extraction,PCR amplification of microsatellite primers, fragment analysis with gel electrophoresis, allele analysis of every locus and calculation of allele frequencies, polymorphism information content （PIC） and heterozygosity. Based on this, the body measurements traits and body weight were used to evaluate their growth and development and the muscularity evaluation traits in the regulation of linear valuation and slaughtering meaty traits were used to evaluate their beef performance traits. The relationship of 21 traits and 8 microsatellite makers was analysised by means of SPSS. 6 microsatellite markers were discovered to have positive or negative correlation with some growth and meaty traits in the population.