复方中草药免疫增强剂对蛋鸡免疫效果的研究

Effects of Compound Chinese Herbal Immune Synergist on Immunity of Egg-laying Chicken

选用3种中草药配方,采用1%、1.5%、2%3个添加水平,进行9个试验组和1个对照组比较研究。对试验鸡的45 d免疫器官质量和T、B淋巴细胞进行检查,对不同日龄鸡新城疫(ND)和传染性法氏囊(IBD)的抗体效价、中性粒细胞吞噬功能和T淋巴细胞转化率进行检测。结果表明,试验组脾质量、法氏囊质量及T、B淋巴细胞相对数高于对照组(P<0.05),以配方B为最高,其次为配方A。试验组抗体效价、中性粒细胞吞噬率、吞噬指数和T淋巴细胞转化率高于对照组(P<0.05),其中ND抗体效价I、BD抗体效价20 d前组间差异不显著,34 d后组间差异趋于显著。54 d和126 d时,A、B、C 3配方中性粒细胞吞噬率和吞噬指数组间差异显著(P<0.05或P<0.01);T淋巴细胞转化率组间也存在极显著差异,其中配方A中的1组具有明显优势。重复试验结果基本相似。

Three Chinese herbal formulations were selected and each fomulation was used at levels of 1 %, 1.5 % and 2 % respectively. Weights of immune organs, B- and T-lymphocytes numbers at 45 days of ages, antibody titers of ND and IBD, phagocytotic function of neutral granular cells and conversion rates of T lymphocytes were measured. The results showed that weights of spleen and bursal, relative numbers of B-lymphocytes and T-lymphocytes in experimental groups were higher than those in control （P〈0.05）, particularly in prescription B group. Antibody titer of ND and IBD, phagocytosis rates and indices, conversion rates of T-lymphocytes in experimental groups were higher than those in control （P〈0.05）. The differences of antibody titer in different groups were not significant before 20d, and tented to be significant after 34 days of age （P〈 0. 01 ）. The differences of phagocytosis rates and indices at 54 and 126 days of age between groups were significant （P〈0.05 or P〈0.01）. High significant differences of conversion rate of T-lymphocytes between groups at 54 and 126 days of ages were also found. Prescription A was better than B and C, among which group 1 showed more obvious effect. The results of repeated experiment were similar to that of the above.