安祖花愈伤组织诱导和植株再生的研究

Induction of Callus and Plantlet Regeneration of Anthurium andraeanum

为建立适合安祖花遗传转化的受体体系,分别选取安祖花组培苗带叶柄的叶片和带叶柄的茎段为外植体,进行了愈伤组织的诱导和植株再生试验。结果表明:以带叶柄的茎段为外植体对愈伤诱导效果好于带叶柄的叶片。使用带叶柄茎段为外植体,以1/2MS+BA 1.0 mg/L+2,4-D 0.1 mg/L为基本培养基进行愈伤组织诱导和继代培养,愈伤组织诱导率最高为89.65%;使用3%蔗糖作为碳源的1/2MS添加0.1 mg/L KT能显著提高诱导率;添加NAA 0.5 mg/L或2,4-D 0.5 mg/L有助于生根。

Induction of callus and plantlet regeneration were carried out from leaves and shoot cuttings with petiole as explants in Anthurium andraeanum. The results indicated that the frequencies of callus formation from shoot cuttings with petiole were higher than those from leaves with petiole. The medium containing 1/2MS ＋ BA 1.0 mg/L＋ 2, 4-D 0.1 mg/L was proved to be the most effective for inducing callus from shoot cuttings with petiole and the highest frequency of callus induction was 89.65 %. The frequency of callus formation was improved when 0.1 mg/L KT, 1/2MS and 3% sucrose were applied. Adding 2, 4-D 0.5 mg/L or NAA 0.5 mg/L benefited root formation. Suitable type of explants was found and the adaptive acceptor system for genetic transformation was also built.