端粒酶活性与宫颈癌病变关系研究及检测

RELATIONSHIP BETWEEN TELOMERASE ACTIVITY AND CERVICAL CANCER

目的探讨端粒酶活性与官颈癌的相关性。方法对30例浸润型宫颈癌，72例宫颈上皮内瘤样病变（CINⅢ28例、CINⅡ24例。CINⅠ20例）厦16例正常者的官颈新鲜组织，用TRAP—PCR检测端粒酶活性。结果宫颈癌组中端粒酶阳性22例（73．33％），CINⅢ级中端粒酶阳性13例（46．4％），CINⅡ级中端粒酶阳性6例（25％），CINⅠ级中端粒酶阳性2例（10．00％）；而16例正常言颈组织仅有1例端粒酶阳性（6．25％），宫颈癌和CINⅠ～CINⅢ组织端粒酶活性表选差异有统计学意义（P〈0．01）。CINⅢ和正常对照组端粒酶活性表选差异有统计学意义（P〈0．01）。结论端粒酶活性在宫颈癌发生发展中可能起到重要作用，在子宫颈病变中检测端粒酶活性对于探讨宫颈病变的进展和宫颈癌前病变的发生具有重要意义。

Objective To study the relationship between telomerase activity and cervical cancer. Methods Telomerase activity in fresh tissues of 30 cases with infiltrating cervical cancer,72 cases with cervical intraepithelial neoplasia（CIN,28 cases CINⅢ ,24 cases CIN Ⅱ ,20 cases CIN Ⅰ ）and 16 cases with normal control cervix were measured by TRAP- PCR. Results There were 22 cases with positive telomerase in cervical cancer tissues（73. 33%）,13 eases in CIN Ⅲ tissues（46. 4%） 6 cases in CINⅡ tissues（25.00%）, 2 cases in CIN Ⅰ tissues（10.00%）, and only 1 ease in 16 normal control tissues. There were significantly higher telomerase activity in cervical cancer and precancerous lesion tissues than those in benign lesion and normal control tissues（P〈0.01）. Conclusion Telomerase activity may play an important role during cervical carcinogenesis, it is signality for cervical disease advancement and cervical carcinogenesis.