玉米赤霉醇人工抗原合成及其多克隆抗体的制备

Synthesis of Artificial Antigen and Preparation of Polyclonal Antibodies Against Zeranol

对玉米赤霉醇（ZER）16-OH进行改造，设计合成了模拟玉米赤霉醇特征结构的半抗原——ZER-16-羧丙基丁醚。用混合酸酐法将其与载体蛋白BSA连接，用于免疫新西兰大白兔，制备的抗血清效价达1：102400。基于活性酯法合成的包被抗原建立了CI-ELISA检测方法，该法对玉米赤霉醇检测的线性范围为18．39～1744．70ng／mL，检出限为8．61ng／mL。

To produce a novel type of immunogen susceptible to raise antibodies, zeranol was converted to zerenol-16-carboxy propyl ether and conjugated to bovine serum albumin （BSA） by the mixedanhydried method. Three New Zealand white rabbits were immunized with ZER-BSA, and the titers of antiserum had reached 1：102 400. A ZER-OVA, based CI-ELISA was conducted with the antiserum, the response range for zeranol was between 18.39-1 744.70 ng/mL,and the detection limit was 8.61 ng/mL.