“分子梳”研究进展

Advances in Molecular Combing

“分子梳”是DNA被可移动的气-液界的均匀拉直,该技术涉及两个过程:DNA分子末端与基底表面的特异性结合和移动的气-液界面对DNA分子的均匀拉直。该技术在构建纳米材料/结构,研究DNA转录和复制,绘制基因物理图谱等方面得到了应用。预计“分子梳”技术将在以下方面取得进展:以拉直的DNA为模板构建纳米器件和纳米材料;用于基因突变的临床检测;结合原子力显微术(AFM),建立“原子力显微术原位杂交”技术以替代荧光原位杂交。

Molecular combing was developed by Bensimon in 1994, which can be applied for stretching long DNA molecules uniformly. Two processes are involved in molecular combing, i. e. , the specific binding of DNA extremities to substrate surfaces and the uniform combing of DNA caused by a moving air-liquid interface. This technique has been applied to constructing nano-materials and nano-structures, studying DNA rep molecular combing cation and transcription, and physical mapping of DNA. It can be expected that s promising in the applications of fabrication of nano-devices using combed DNA as template, determination of gene mutation, and establishment of a new method of ＂atomic force microscopic in-situ hybridization＂ to replace the fluorescence in-situ hybridization.