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人Lrg在真核细胞内亚细胞定位的检测 被引量：1

The Intercellular Localization of Human Lrg in Eukaryotic Cells
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摘要 在真核细胞内,进行人Lrg蛋白亚细胞定位的检测。采用绿色荧光蛋白载体pIRES2-EGFP,构建pIRES2-EGFP-Lrg重组载体,稳定转染到真核细胞内,荧光显微镜进行人Lrg蛋白亚细胞定位的检测;同时利用间接免疫方法,检测人Lrg蛋白在几种细胞内的亚细胞定位。发现与EGFP融合的人Lrg蛋白表达于胞浆;间接免疫荧光实验同样表明Lrg是一种胞浆蛋白。说明人Lrg蛋白可能定位于胞浆。上述结果为阐明人Lrg的功能奠定了初步基础。 In order to analyze the intercellular localization of human Lrg, eukaryotic expressive vector of EGFP-HLrg fusion protein named pIRES2-EGFP-HLrg was constructed. Then transfected into HEK293 by way of lipofectin. The intercellular localization of human Lrg was further analyzed by indirect immunofluorescence technique. Fluorescence microscopy analysis is revealed that human Lrg localized in cytoplasm mainly. The results also demonstrated that human Lrg localized mainly in cytoplasm of HepG2 and Dental Papilla cells. It is concluded that human Lrg could be detected in cytoplasm of several cells. These results lay the foundation for the further study of human Lrg.
作者 杜可军 常文辉 侯立朝 骆文静 刘承利 陈苏民 陈景元 柴玉波
机构地区 第四军医大学劳动与环境卫生教研室 第四军医大学生物化学和分子生物学教研室
出处 《科学技术与工程》 2006年第1期13-16,共4页 Science Technology and Engineering
基金 国家自然科学基金项目(30170361 30400413 30471675) 第四军医大学博士后基金(00001149)资助
关键词 人Lrg蛋白 EGFP 间接免疫荧光 胞内定位 HLrg protein EGFP indirect immunofluorescence technique intercellular localization
分类号 Q271 [生物学—细胞生物学]
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参考文献10

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同被引文献2

  • 1Spiers A J, Rainey PB. The Pseudomonas fluorescens SBW25 wrinkly spreader biofilm requires attachment factor, cellulose fibre and LPS interactions to maintain strength and integrity [ J ]. Microbiology, 2005, 151 (9) : 2829 -2839.
  • 2Schippers EF, van't Veer C, van Voorden S, et al. IL-10 and toll- like receptor-4 polymorphisms and the in vivo and ex vitro response to endotoxin[J]. Cytokine, 2005, 29(5) : 215 -228.

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科学技术与工程
2006年 第1期

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