胚胎发育相关基因精子转录本在白血病细胞系中的表达及其调节

Expression of distal (testis) promoter of EDAG in leukemia cell lines and its regulation during cell differentiation

目的探讨胚胎发育相关基因(EDAG)在白血病细胞中的调控机制。方法用半定量RT-PCR方法检测白血病细胞系中EDAG精子转录本EDAG-t的表达;使用不同的分化诱导剂诱导K562和U937细胞分化,检测EDAG-t的表达改变情况。结果在白血病细胞系K562和U937中也可检测到EDAG-t的表达;使用不同的分化诱导剂诱导K562和U937细胞分化,发现EDAG精子转录本和造血转录本的表达都会随细胞分化而下调,但它们的下调步伐不一致,总的说来,EDAG-t的减弱较EDAG-h慢。结论EDAG精子转录本在白血病细胞中的异常表达值得进一步研究。

Objective To explore the regulation mechanism of EDAG expression in leukemia cells. Methods We detected the pattern of EDAG-t mRNA expression via RT-PCR in a panel of leukemia cell lines. Then we further explored the regulation of EDAG-t and EDAG-h expression during the differentiation of K562 and U937 cells. Results EDAG-t （human HEMGN-t） isoform could also be detected in the leukemia cell lines K562 and U937. And both HEMGN-t and HEMGN-h could be down regulated during the differentiation of K562 and U937 cells but the kinetics were different. Conclusion The regulatory mecha- nisms for HEMGN-t in testis and leukemia cell lines deserve further investigation.