幼胚胚性愈伤组织诱导的研究

Induction of Embryoic Calli from the Young Embryos of Nai(Prunus salicina Lindl.var.cordata J.Y.Zhang et al.)

试验以油（木奈）幼胚为材料，研究外植体大小、低温处理、基本培养基类型、碳源、培养基中的2，4-D浓度、AgNO3的添加与否对胚性愈伤组织诱导的影响，结果表明，长轴为5—7．9mm低温处理18h的幼胚，诱导胚性愈伤组织的效果最好；使用山梨醇（浓度为3％）为碳源、含氮量低的WPM培养基，胚性愈伤组织的诱导效果优于蔗糖为碳源、含氮量高的MS培养基；幼胚胚性愈伤组织诱导的最适2，4-D浓度为2．0mg／L，培养基中附加5．0mg／LAgNO3有助于提高胚性愈伤组织的诱导率。

The effects of sterilizing condition, the size of explant, treating time under low temperature, the types of basic media, carbon resources, the concentrations of 2,4 - D, addition or no addition of silver nitrate in media, and other culture conditions on the induction of calli were probed with the young embryos of Nai as the explant. The results showed that the optical sterilizing condition was that the immature embryos were dipped in 0.1% HgCl2 solution for 8 min ; the young embryos ranged from 5 mm to 7.9 mm in long axis treated under low temperature for 18 hours were optical to the induction of embryonic calli; the induction effects of WPM medium with sorbitol as carbon resource were superior to MS medium with sugar as carbon resource; the optical concentration of 2,4 - D in media was 2.0 mg/L; adding 5 mg/L silver nitrate was benificial to raise the induction rate of embryonic calli; dark culture was adopted in the whole process of induction of calli.