快速检测金黄色葡萄球菌肠毒素A基因方法的建立与应用

Establishment and application of a rapid precise method to detect Staphylococcus aureus enterotoxins A

目的建立一种快速准确定量检测金黄色葡萄球菌肠毒素A的方法。方法以femB、SEA基因分别作为金黄色葡萄球菌菌株、肠毒素A的靶序列，设计合成引物和TaqMan探针；收集腹泻患者大便标本分离的金黄色葡萄球菌68株，并定量检测其肠毒素A。结果TaqMan探针荧光聚合酶链反应检测金黄色葡萄球和肠毒素A的灵敏度均为1．0×10^2拷贝。68株金黄色葡萄球菌中检出产肠毒察A菌株11例（16．2％，11／68），CT值为13．5—20．6。结论TaqMan探针荧光聚合酶链反应能够准确快速检测金黄色葡萄球菌肠毒素A。

Objective To establish a rapid specific quantitative assay for the detection of Staphylococcus aureus enterotoxins A. Methods Design and synthesize the primers and TaqMan probe targeted at the genes of femB and SEA. 68 isolates of Staphylococcus aureus originating from stool smaples were analyzed. Results The sensitivity of the assay for Staphylococcus aureus enterotoxins A was 1.0 × 10^2 copies. Among 68 samples, 11 strains were positive for enterotoxins A （ 16. 2%, 11/68）. Conclusion The TaqMan PCR could rapidly exactly detect the Staphylococcus aureus enterotoxins A.