HVA1基因转化番茄及转基因番茄耐盐性的初步鉴定

Transformation of HVA1 Gene into Tomato and Elementary Identification of Salt-tolerance

本研究将含有HVA1基因的质粒pBY520与pCAMBIA2200构建成双元载体pH22,又在pBY520上加上MAR元件RB7后,再与pCAMBIA2200构建成双元载体pHR22。并将上述两个载体通过农杆菌介导转入番茄,获得24株转化植株,并经过PCR-Southern检测证明其中11株为转基因阳性,且均表现出一定的耐盐性,表明HVA1基因已被转入这些植株中。

The binary vector pH22 was constructed using pBY520 harboring HVA1 gene and pCAMBIA2200 as basic plasmids. Then, pBY520, appended MAR element RB7 to, and PCAMBIA2200 were used as construction of the binary vector PHR22. The veeto,＇s of pH22 and pHR22 were transferred into tomato mediated by Agrobacterium tumefaciens and 24 transgenic plants were obtained. 11 of them showed positive signals by PCR-Southern identification and salt-tolerance, which indicated that HVA1 gene had been successfully introduced and integrated into the genome of these 11 transgenic plants.