半套式PCR方法检测猪附红细胞体病的建立

Establishment of Half-nested PCR Method on Eperythrozoon Diagnosis

建立一种客观敏感的附红细胞体病早期实验室诊断方法。本实验采用已公布的附红细胞体序列设计半套式引物进行PCR扩增,对扩增出得特异性条带进行序列分析。镜检结果阳性率为75%,PCR检测结果阳性率为90%,测序结果与已公布的序列同源性达99.9%。半套式PCR方法比镜检高效准确,适合附红细胞体病的早期诊断。

Establishing an efficient method for eperythrozoonosis diagnosis. In this study, half-nested PCR was performed to detect the samples after microscopic examination. The result of microscopic examination and half-nested PCR showed the infection rate was 75 % and 90 % respectively, which demonstrated that PCR was the best way for early and chronic eperythrozoonosis diagnosis.