摘要
目的探讨压力超负荷状态下左室心肌牵张敏感性钾通道TREK-1mRNA及其蛋白表达的变化。方法雄性W istar大鼠被随机分为腹主动脉缩窄组(n=30)和假手术组。腹主动脉缩窄组依观察时间随机分为2,4,8周组各10只。每组观察期结束后,计算左右室肥厚指数(LVM I、RVM I)。HE染色观察压力超负荷对心肌组织结构的影响。采用RT-PCR和免疫组织化学SABC法分别检测牵张敏感性钾通道TREK-1mRNA与蛋白水平表达的改变。结果腹主动脉缩窄术后2周,出现左室肥厚,但无统计学意义。腹主动脉缩窄术后4,8周,左室肥厚指数较正常组明显增加(P<0.05),光镜下显示心肌肥厚。术后2,4,8周组左室心肌牵张敏感性钾通道TREK-1mRNA上调(P<0.05),心肌细胞内TREK-1蛋白表达明显增加(P<0.05)。结论压力超负荷致大鼠左室肥厚的同时可诱导牵张敏感性钾通道TREK-1的表达增加。
Objective To investigate expression changes of stretch-activated potassium channel TREK-1 mRNA and protein induced by coarctation of abdominal aorta (CAA). Methods Male Wistar rats were randomly divided into four groups:CAA2w ,CAA4w,CAA8w, and sham-operated group(n = 10 in each group). Left and right ventricular mass index (LVMI,RVMI) ,cardiac morphological changes were investigated in different groups. The expression of TREK-1 mRNA was examined by semi-quantitative RT-PCR, and the expression of TREK-1 protein was detected by immunohistochemistry. Results Compared to sham-operated group,left ventricles became hypertrophy at 2 weeks after coarctation of abdominal aorta,but it had no statistical significance;Left ventricular mass indes(LVMI) increased significantly at 4 and 8 weeks after abdominal coarctation(P〈0.05), and hypertrophic cardiomyocytes were seen, under optioal microscope. TREK-1 mRNA strongly expressed and protein up-regulated in groups CAA2w, CAA4w and CAA8w ( P 〈0. 05). Conclusions Expression of TREK-1 up-regulated during the progression of left ventricular hypertrophy induced by chronic pressure overload.
出处
《中国心脏起搏与心电生理杂志》
2006年第1期69-71,共3页
Chinese Journal of Cardiac Pacing and Electrophysiology
基金
国家自然科学基金资助项目(批准号:30270559)
关键词
心血管病学
压力超负荷
牵张敏感性钾通道
左室
腹主动脉缩窄
Cardiology
Pressure overload
Stretch-activated potassium channel
Left ventricle
Coarctation of abdominal aorta