自动生化分析仪检测人抗凝血酶Ⅲ及其临床应用探讨

The Clinical Role of Antithrombin Ⅲ Detection Using the Automatic Biochemical Analyzer

目的抗凝血酶Ⅲ对机体凝血和纤溶系统的调控有重要的作用。本文用自动生化分析仪检测人抗凝血酶Ⅲ，使抗凝血酶Ⅲ的检测具快速、准确，且成本大为下降。方法抗凝血酶Ⅲ抗体与人体中抗凝血酶Ⅲ结合产生免疫浊度，根据浊度的不同使用自动生化分析仪检测抗凝血酶Ⅲ浓度。肝硬化患者100例、急性心梗患者40例、外科术后患者50例、凝血酶原时间延长患者50例，与正常对照组100例分别检测抗凝血酶Ⅲ浓度。结果经t检验统计处理，正常对照组与上述各患者组均有显著性差异（P〈0.01）。结论抗凝血酶Ⅲ测定对凝血和纤溶系统疾病的诊断及治疗中的监测有重要的作用。

Objective AntithrombinⅢ plays an important role in the regulation of clotting and fibrinolysis system in human beings. The detection of antithrombinⅢ became more quick, accurate and cheap using the automatic biochemical analyzer. Methods Antithrombin Ⅲ assay is based on the measurement of immunoprecipitation. Antibodies against human antithrombin Ⅲ undergo an agglutination reaction with antithrombin Ⅲ, resulting in an increase in the turbidity of the mixture. The concentration of antithrombin Ⅲ was detected in 100 patients of liver cirrhosis, 40 patients of acute myocardial infarction, 50 post-operation patients, 50 patients with prothrombin time elongation and 100 health controls using the automatic biochemical analyzer. Results The significant differences of concentration were found between all cases and controls using the t test（P〈0.01）. Conclusion The progress monitoring of clotting and fibrinolysis diseases can benefit from antithrombin Ⅲ observation in human beings.