化疗药物与LAK细胞联合抗癌的可行性

Feasibility of Combined Anticancer with Chemotherapeutic Drugs and IL-2/LAK cells

采用３Ｈ－ＴｄＲ释放法检测６种常用化疗药物对ＬＡＫ细胞杀瘤活性的影响。结果显示：不同化疗药物对ＩＬ－２诱导ＬＡＫ细胞活性及对培养好的ＬＡＫ细胞活性的影响各不相同，同种化疗药物不同浓度时的影响也有差异。卡铂（Ｃａｒｂｏ）和顺铂（ＣＤＤＰ）对ＩＬ－２诱导ＬＡＫ细胞活性无抑制作用，且可增强培养好的ＬＡＫ细胞的杀瘤活性；环磷酰胺（ＣＴＸ）和５－氟尿嘧啶（５－Ｆｕ）对诱导及培养好的ＬＡＫ细胞活性均无抑制作用；阿霉素（ＡＤＭ）和丝裂霉素（ＭＭＣ）对诱导及培养好的ＬＡＫ细胞活性均有抑制作用。由此推论Ｃａｒｂｏ和ＣＤＤＰ与ＩＬ－２／ＬＡＫ细胞联合抗癌可望产生协同效应；ＣＴＸ和５－Ｆｕ与ＩＬ－２／ＬＡＫ细胞联合抗癌可能有相加作用；而ＡＤＭ和ＭＭＣ则可能削弱ＬＡＫ细胞的杀瘤效应。

Kinds of chemotherapeutic drugs often used mere studied by 3H-TdR releaseassays to detect their effects on IL-2/LAK activity. The results showed that:different chemotherapeutic drugs's effects on IL-2/LAK activity was different, and different concentrations of the same chemotherapeutic drug's was different also. Carbo/CDDP had no inhibitory effects on induction of LAK activity by IL-2, and couldaccelarate the lysis activity of LAK cell; CTX/5-Fu had no inhibitory effects onIL-2/LAK activity; But ADM/MMC had inhibitory effects on IL-2/LAK activity.These suggested that: Carbo/CDDP combined with IL-2/LAK in cancer treatmentmay have cooperations; CTX/5-Fu combined with IL-2/LAK may have plus actions; But ADM/MMC may reduce LAK activity.