白血病中IL－2受体基因的表达及其意义

The Expression of IL-2 Receptor gene and its significance in leukemia

利用聚合酶链反应（ＰＣＲ）方法检测１１６例各种白血病细胞ＩＬ－２受体基因的表达情况。该基因在Ｔ－ＡＬＬ、ＣＬＬ、Ｃ－ＡＬＬ和ＡＮＬＬ都有相当高的表达率，分别为１００％、１００％、７１．４３％和６５％。该方法较ＡＰＰＡＰ和免疫荧光法发现更高的阳性率，提示其敏感性及准确性更高。本文结果显示了大部分的白血病细胞都表达了ＩＬ－２受体基因，推测ＩＬ－２受体可能是白血病细胞所具有的一种普遍特性，而ＩＬ－２则可能在白血病细胞的增殖中起一定的作用。故提出临床上应重新评价ＬＡＫ细胞和ＩＬ－２在白血病中的治疗价值。

The expression of IL-2 receptor gene in 116 cases with leukemia was analysed using polymerase chain reaction (PCR)method. The expression rates were highly in T-ALL, CLL, c-ALL and ANLL, 100%, 100%, 71. 43% or 65%,respectively. More positiv cases could found with PCR method as compared with APPAP or immunofluorescence analysis. Suggesting that it had more sensitive and accurate. Our data indicated that the most leukemia cells expressed the IL-2 receptor gene and that IL-2 receptor would be a common feuture of leukemia cells and suggest that IL-2 may be play a certain role in proliferation of leukemia cells.Therefore, it may be to reevaluate the therapeutical value of LAK cells and IL-2 by leukemia in clinic.