癌旁组织增殖特性的流式细胞测量术分析

Flow cytometric Study of Ploliferous Characteristics in Para-cancer Tissue

作者用流式细胞测量术对３０４例常见恶性肿瘤患者癌旁组织增殖特性进行了研究。结果表明，癌旁组织Ｓ期细胞比率（ＳＰＦ）和超二倍体细胞比率（＞２Ｃ％）均显著高于正常对照组织（Ｐ＜０．０１），与癌中心灶十分接近。癌旁组织的ＤＮＡ倍体类型不同，其＞２Ｃ％明显不同，异倍体者显著高于二倍体（Ｐ＜０．０５）；癌旁组织倍体类型对其ＳＰＦ影响不明显。癌中心灶ＤＮＡ倍体类型对癌旁组织的＞２Ｃ％和ＳＰＦ未见明显影响。随肿瘤体积增大，癌旁组织的ＳＰＦ有逐渐减低的倾向，其中肝癌旁组织表现最明显（ｒ＝－０．９６８，ｒ＞ｒ０．０１）；随肿瘤增大，癌旁组织超四倍体细胞比率（＞４Ｃ％）呈递增的变化（ｒ＝０．８４，ｒ＞ｒ０．０５）。

Proliferous Characteristics were measured on 304 samples of para-cancer tissue in diseases with common malignant tumor. The results showed that SPF (S-phase fraction) and 2C% (high diploidy percentage) of para-cancer tissue were higher than control (p<0.01). SPF and 2C% of para-cancer tissue were approximate to cancer nests. Frequency of > 2C% in para-cancer tissue was difference of itself ploidy types.>20% of aneuploidy tumor was higher than that of diploidy tumor (p<0. 05). The frequency of SPF in para-cancer tissue not affected by itself ploidy types. The frequency of>2C% and SPF not affected by ploidy types of cancer nests. The frequency of>2C% para-cancer tissue not observed.regular change, while that of SPF was a tendency of dicrease with increment of volume in cancer. change of hepatocellular cancer was evidentest in all cancer (r=- 0. 968, r>r 0. 01). Percentage of high ploidy cells increased with increment of volume in cancer (r=0. 84, r>r 0. 05).