大豆总异黄酮HPLC指纹图谱研究

Fingerprints of soybean total isoflavone by HPLC

目的建立不同品种大豆植物药材异黄酮类成分的HPLC指纹图谱,为科学评价大豆品种内在质量提供参考。方法采用HPLC法测定了25个不同品种大豆的异黄酮类提取物,并用聚类分析和相似度计算软件进行数据分析。色谱条件:C18柱(250 mm×4.6 mm,5μm),流动相为甲醇-水(冰醋酸调pH为3.2)梯度洗脱,体积流量为0.6 mL/m in,检测波长为261 nm,柱温为室温。测定了25个不同品种大豆,运用软件进行聚类分析及相似性分析。结果该方法重现性好,不同品种大豆提取的异黄酮类成分的色谱指纹图谱有一定的差异。结论色谱指纹图谱分析方法能够全面反映出不同品种大豆异黄酮化学成分间的差异,为大豆品种内在质量控制提供参考。

Objective To study the HPLC fingerprints of isoflavones from different medicinal plants of G. max and establish a sensitive and specific method for controlling the quality of the soybean isoflavone. Methods The gradient elution mode was applied in chromatographic separation. A C18 column （250 mm×4.6 mm, 5μm） was used with the mobile phase, ice acetic acid water solution （pH value 3.2）-methanol, flow rate. 0. 6 mL/min, detecting wavelength. 261 nm, and the column temperature, room temperature. All 25 samples collected from different species were determined. The clustering analysis and the software of similarity analysis were applied for datum analysis. Results This method had a good repeatability and reproducibility. The ratio of peaks/ area from distinct samples were different. Conclusion The method can show the difference of chemical compositions among species completely and can be used as a quality control method for soybean.