正交试验法优选繁枝蜈蚣藻粗多糖的提取工艺

Optimum Extraction Process of Polysaccharides from Grateloupia filicina by Orthogonal Test

本文对繁枝蜈蚣藻中可溶性粗多糖的提取工艺进行了研究,通过单因素试验和L9(33)正交试验,研究了料液比、温度、时间对多糖提取率的影响,结果显示温度和料液比是影响多糖提取率的主要因素,最佳工艺为料液比1:60,温度100℃,时间3h,在最佳提取工艺时,繁枝蜈蚣藻的多糖提取率为22.527%。采用紫外检测和茚三酮法检测未见多糖含有核酸、蛋白质。

This paper studied the extraction process of polysaccharide from Grateloupiafilicina. Single factor test and orthogonal experiment design methods L9（3^3） were applied to analyze the effects of the factors such as solid-liquid ratio and temperature and time on the extraction percentage of the polysaccharides. Experimental results indicated that the temperattLre and solid-liquid ratio would significantly affect the extraction percentage of the soluble polysaccharides. The optimum solid-liquid ratio was： 1：60, the temperature 100℃ and the extraction time 3h. In the conditions, the extraction percentage of soluble polysaccharides in Grateloupiafilicina was 22.527%. No nuclear-acid and protein has been found in the polysaccharides by ultraviolet detection and Ninhydrin reaction,