超滤在大豆多肽分离纯化中应用

Application of Ultrafiltration on Separation and Purification of Soybean Peptides

本试验应用超滤技术对大豆多肽进行分离,研究了超滤系统几个主要参数对膜通量的影响。研究结果表明:截留分子量30000Da的超滤膜应在压力0.12MPa、温度40℃、pH值7.0下运行40min为一个周期,膜通量为19L/m2·h左右;截留分子量10000Da的超滤膜应在压力0.10MPa、温度<45℃、自然pH值下运行60min为一个周期,膜通量为25L/m2·h左右;截留分子量5000Da的超滤膜应在压力0.10MPa、温度<45℃、自然pH值下运行80min为一个周期,膜通量为22L/m2·h左右;经分离得到分子量>30000Da的大豆多肽约占13.21%,分子量10000～30000Da的大豆多肽约占4.05%,分子量5000～10000Da的大豆多肽约占6.41%,分子量<5000Da的大豆多肽约占76.11%。

In this experiment, soybean peptides were separated by ultrafiltration. How those major ultrafiltration-system parameters affecting the membrane flux were studied. It has showed that the ultrafiltration membrane with 30000 MWCO （Molecular Weight Cutoff） should run 40min for a cycle at 0.12MPa, 40℃ and pH7.0 and under this condition, the membrane flux would be about 19L/m^2·h; when the ultrafiltration membrane with 10000 MWCO should run 60min for a cycle at 0.10MPa, 〈45℃ and natural pH and under this condition, the membrane flux would be about 25L/m^2·h; when the ultrafiltration membrane with 5000 MWCO should run 80min for a cycle, at 0.10MPa, 〈 45℃ and natural pH and under this condition, the membrane flux would be about 22L/m^2·h. In the end, soybean peptides were separated according to its molecular weight： 13.21% with molecular weight 〉30000Da; 4.05% with molecular weight 10000-30000Da; 6.41% with molecular weight 5000-10000Da; and 76.11% with molecular weight 〈 5000Da.