摘要
本文研究了高效液相色谱仪测定甜菜红色素含量的方法。采用Shim-packvp-ODS色谱柱(150mm×4.6mmi.d.,5μm),流动相为A:甲醇、B:2%冰醋酸缓冲液(A:B=20:80V/V),流速0.7ml/min,检测波长为535nm。甜菜红色素质量浓度在0.0641～3.206mg/ml范围内与峰面积呈良好线性关系,相对标准偏差为1.13%。,回收率为97.58%～102.10%。本方法操作简便、灵敏、可靠、适用于甜菜红色素的分析测定。
A method for determination of betalain was described by high performance liquid chromatrgraphy. Shim-pack vp- ODS colunm（4.6mm i.d.×150mm, 51am）was used and mobile phase was MeOH：H2O-HAc（20： 80） with flow rate of 0.7ml/min. The eluate was monitored at λmax =535nm. The lineadty was obtained over the range of 0.0641-3.206mg/ml （ r = 0.9999）.The determination limit was 0.0641μg/ml.The average recovery of sample was in the range of 97.58%- 102.10%, with relative standard deviation of 1.13%. The method was proved to be a simple, rapid and reliable way to determine the betalain concentration.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2006年第2期215-217,共3页
Food Science
基金
国家863计划项目(2001BA804A21)
关键词
高效液相色谱
甜菜红色素
测定
high-performance liquid chromatrgraphy
betalain
determination
