聚丙烯酰胺凝胶电泳研究猪血清蛋白硫酸铵分级盐析

Study of Ammonium Sulfate Fractionation of Porcine Serum Protein by Polyacryamide Gel Electrophoresis

本文通过聚丙烯酰胺凝胶电泳研究了盐析时各种猪血清蛋白溶解度与硫酸铵饱和度的关系,结果显示猪血清蛋白析出区间为25%～70%硫酸铵饱和度,各种猪血清蛋白具有各自的沉淀区间。10倍稀释猪血清白蛋白最佳硫酸铵沉淀方案为:pH值7.4,调节硫酸铵饱和度到50%,离心除去沉淀,调清液pH值至4.5,硫酸铵饱和度至55%,沉淀白蛋白。pH值7.4,35%饱和度硫酸铵沉淀猪血清时,IgG得率为69.5%,纯度为76%;40%饱和度时,IgG得率为91.8%,纯度为61.6%。

This paper studied the relation between porcine serum protein and ammonium sulfate saturation when salt fractionation by Polyacryamide Gel Electrophoresis, the result showed the main deposition scope of porcine serum protein is 25%-70% ammonium sulfate saturation, each porcine serum protein has respective deposition scope. The best ammonium sulfate fractionation method of decuple dilute porcine serum albumin is pH7.4, adjust ammonium sulfate saturation to 50%, remove deposition by centrifugation, and adjust the pH of clear liquid to 4.5, the ammonium sulfate saturation to 55% ,then albumin was deposited. In the porcine serum protein which was deposited by 35% saturation of ammonium sulfate when pH is 7.4, the IgG yield rate is 69.5% and purity is 76%, when the ammonium sulfate saturation is 40%, the IgG yield rate is 91.8% and purity is 61.6%.