摘要
在本项研究中,应用反相C18高压液相色谱柱对番茄果实中的番茄红素(Lycopene)进行了分离。色谱条件为:C18固定相:DIAMONSILTM柱;流动相A:乙腈:水=9:1;流动相B:乙酸乙酯;线性梯度洗脱:在前15min内,B由0增为100%,随后,B保持100%;流速为1.0ml/min,紫外—可见光检测器波长范围:260～600nm;监测波长:475nm;进样量为20μl;柱温:室温。经过对漂移管温度,载气压力及喷嘴温度三参数的摸索,确定蒸发光散射检测器(ELSD)在检测样品中番茄红素含量时的条件为:漂移管温度:45℃;载气压力:30psi;喷嘴温度:40℃;增益:1。以番茄红素纯品为参比样品,比较了ELSD与经常使用的紫外—可见光(UV-VIS)检测器在对番茄红素定量检测时的结果。结果表明:虽然ELSD的灵敏度不及UV-VIS检测器,但完全可以在番茄红素的定量检测中应用。同时,ELSD可以检测出样品中不带发光基团的其它组分,从而在检测萃取物及产品纯度时有良好的应用前景。
In this study, lycopene from tomato fruits was separated on HPLC by reversed phase C18 column under the conditions as： C18 stationary phase-DIAMONSILTM column mobile phase-A ratio of acetonitrile to water （9：1） and mobile phase-B ethyl acetate; linear binary elution gradient： mobile phase B increased from 0 to 100% in the first 15 minutes and later kept 100% consequently; flow rate-1.0 ml/min; UV-VI detection wavelength 260-600nm; monitoring wavelength-475nm; column temperature-ambient temperature. The detection conditions applied for this sample of evaporative light scattering detector （ELSD） were： drift temperature-45℃; cartier gas pressure-30psi; sprayer temperature-40℃; and gain- 1. After variation in drift temperature, carder gas pressure and sprayer temperature, the comparison of quantitative results from ELSD and UV-VIS detector was made with the reference of pure lycopene. Data from the comparison of results suggested that ELSD can be applied in lycopenequantification although its sensitivity is poorer than that of UV-VIS detector. Additionally, ELSD is cap able to exhibit information on colorless components from the sample and therefore has a bright future in the application of purity check for the extracts and products of lycopene.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2006年第2期226-229,共4页
Food Science
基金
"十五"国家重大科技专项(2001BA804A21)
