摘要
目的探讨香烟烟雾(cigarette smoke,CS)及银杏叶对哮喘大鼠气道组织转化生长因子-β1(TGF-β1)mRNA和蛋白表达的影响。方法30只Wistar大鼠随机分为5组:1)哮喘烟熏组(A组);2)哮喘组(B组);3)哮喘烟熏+银杏叶治疗组(C组);4)哮喘+银杏叶治疗组(D组);5)对照组(E组)。用蛋白印迹法(Western blot)及反转录-聚合酶链反应(RT-PCR)检测各组气道组织中TGF-β1蛋白和mRNA表达的变化。结果香烟烟雾可显著增加哮喘大鼠气道组织TGF-β1mRNA和蛋白的表达,银杏叶则可显著抑制其表达。结论香烟烟雾使哮喘大鼠气道组织TGF-β1的表达上调,提示银杏叶可能通过降低TGF-β1的表达发挥抑制哮喘的作用。
Objective To investigate the influence of cigarette smoke and ginkgo to the expression of transform growth factor-β1 in asthmatic rats. Methods 30 Wistar rats were divided into an asthmatic + CS group(group A), an asthmatic group(group B), an asthmatic+ CS+ ginkgo group(group C), an asthmatic + ginkgo group(group D) and a control group(group E). The expression of TGF-β1 of bronchial tissue in each group was observed by Western blot and reverse transeription-polymerase chain reaction(RT-PCR), respectively. Results The expression of TGF-β1 from group A and B were significantly increased compared with those of group C, D and E correspondingly and respectively(P〈0.01 ). The expression of TGF-β1 from group D was not increased compared with those of group E (P 〉 0.05). Conclusion CSE can increase the expression of TGF-β1 of bronchial tissue whereas ginkgo can decrease the expression of TGF-β1 of bronchial tissue in asthmatic rats.
出处
《首都医科大学学报》
CAS
2006年第1期20-23,共4页
Journal of Capital Medical University