氧化苦参碱对哮喘小鼠的抗炎作用及对ICAM-1 mRNA表达的影响

Effects of Oxymatrine on Anti-inflammatory and ICAM-1 mRNA Expression in Asthmatic Mice

目的探讨氧化苦参碱治疗哮喘的抗炎作用机制及其对细胞间黏附分子-1(ICAM-1)mRNA表达的影响。方法建立小鼠哮喘模型,观察氧化苦参碱对哮喘小鼠支气管肺泡灌洗液中细胞总数、肺组织病理的影响,并应用半定量反转录-聚合酶链反应法检测肺组织中ICAM-1 mRNA表达的变化。结果1)氧化苦参碱可显著减轻哮喘小鼠气道及肺组织中炎性细胞的浸润;2)氧化苦参碱显著抑制哮喘小鼠肺组织中ICAM-1 mRNA表达,此作用与其降低气道炎性细胞的浸润有关。结论氧化苦参碱对哮喘小鼠有明显的抗炎及抑制其ICAM-1 mRNA表达的作用。

Objective To explore the mechanism of oxymatrine anti-inflammatory and on ICAM-1 mRNA expression. Methods Established a murine model of asthma, investigated the effects of oxymatrine on the total cell number of bronchoalveolar lavage fluid （BALF） and histologic examination, and ICAM-1 mRNA expression by semiquantitative reverse transcription-polymerase chain reaction （RT-PCR） in asthmatic mice. Results 1） Oxymatrine can significantly reduced the total cell number of BALF and markedly improved peribronchial eosinophilic infiltration; 2） ICAM-1 mRNA expression was significantly inhibited by oxymatrine, and this may contribute to its anti-inflammatory effects. Conclusion Oxymatine can significantly anti.inflammatory and markedly inhibited ICAM-1 mRNA expression in asthmnatic mice.