实验性变应性鼻炎中鼻黏膜组胺的测定

Measurement of Histamine in Nasal Mucosa in Guinea Pig with Allergic Rhinitis

目的观察变应性鼻炎(AR)动物鼻黏膜中组胺含量的变化,探讨组胺在变应性鼻炎诊断中的价值。方法对影响组胺含量测定的2个因素温度和萃取时间分别进行2个水平考察,以回收率为指标,确定Shore荧光法测定条件;另采用甲苯-2,4-二异氰酸酯(TDI)滴鼻致敏豚鼠变应性鼻炎模型,改进Shore荧光法测定豚鼠鼻黏膜组胺含量,并与对照组进行比较。结果在室温和萃取时间为45 min条件下的组胺回收率满足要求,利用该法测得自然对照组和变应性鼻炎组鼻黏膜组胺含量分别为(513.7±49.3)ng/g和(590.4±47.6)ng/g,变应性鼻炎组鼻黏膜中组胺含量显著高于自然对照组(P<0.01)。结论改进Shore荧光法测定鼻黏膜中组胺含量准确可靠,检测鼻黏膜中组胺含量的高低对诊断变应性鼻炎具有重要价值。

Objective To observe the content of histamine in nasal mucosa in allergic rhinitis（AR） guinea pig, and study the role of it in the diagnose of AR. Methods In the Shore fluorescent method, two factors： temperature and extraction time that affected the measurement of the histamine were estimated respectively at two levels by the recovery rate of histamine to build the final method of measurement of histamine. In addition, the allergic rhinitis models in guinea pig were built by using sensitizing agent TDI, and the histamine in nasal mucosa in guinea pigs were measured by the method built above. Results At the condition of 25℃ and 45℃min of extraction time, the recovery rate of histamine measured up the requirement. And by the method, the histamine content in nail mucosa in controlled group and AR group were （513.7±49.3）ng/g and （590.4 ±47.6）ng/g respectively. There were significant difference between natural controlled group and AR group（ P〈 0.01 ）. Conclusion The improved Shore fluorescent method could accurately and reliably measure the histamine in nasal mucosa in guinea pig. The histamine content in nasal muccosa played an important role in diagnosing AR disease.