弱精子症患者精核蛋白表达的检测及意义

Analysis of Protamine mRNA in sperm of asthenozoospemia patients

目的研究不育症患者精核蛋白mRNA表达量的变化及意义。方法提取6例不育症患者及5例健康男性精子的总RNA,应用RT-PCR法扩增得到精核蛋白cDNA片段;提取精子总碱性核蛋白并进行SDS-聚丙酰胺凝胶电泳,检测其中精核蛋白及组蛋白的含量。结果精子中存在精核蛋白基因的mRNA;不育症患者精核蛋白的含量及精核蛋白mRNA相对含量均下降。结论精核蛋白mRNA相对含量的变化与其蛋白含量的变化一致,可能反映了精子发生过程中某些基因的表达情况。

Objective To investigate the expression of protamine in sperm of infertile men. Methods Total RNA was isolated from sperm of infertile men. By means of RT-PCR techniques, protamine cDNA fragments were obtained from total RNA and relative amount of protamine mRNA was studied. Total basic nuclear protein was extracted and the content of protamine was analyzed by SDS-PAGE electrophoresis. Results There was expression of protamine mRNA gene in spermatozoon. The relative amount of both protamine protein and its mRNA is abnormal in infertile men. Conclusion The change of protamine mRNA relative amount was consist with that of protamine protein in infertile men, and both protamine mRNA and protein may reflect the condition of corresponding gene expression during spermatogenesis.