摘要
目的探讨12例伴有性染色体丢失的t(8;21)急性髓系白血病患者的临床和生物学特征。方法结合临床、形态学,采用RHG显带、一组单抗、逆转录-聚合酶链反应等方法对其染色体、免疫分型和分子生物学等进行分析。结果本组病例大多有下述形态学改变:白血病细胞有核凹陷、近核浅染区、胞浆嗜碱性、伴有成熟分化和核浆发育不平衡等;有CD13、CD33表达抗原;经逆转录-聚合酶链反应检测均检出AML1-ETO融合基因转录本。结论性染色体丢失是t(8;21)急性髓系白血病最常见的附加异常,主要和急性粒细胞白血病有关,具有独特的形态学、免疫学和临床特征。
Objective To explore the clinical and biological characteristics of 12 cases of t ( 8 ; 21 ) acute myeloid leukemia with sex chromosome loss. Methods An comphrehansive analysis including clinical information, cell morphology,chromosome,immunophenotype and molecular biologywas performed in 12 cases of t (8 ; 21 ) AML with sex chromosome losing. Results Most of them had the morphological changes and cytoplasm,, megaloblastoid changes and nuclear-cytoplasm imbalance, the expression of CD13 and CD33 antigen, and the AML1/ETO fusion transcript examined by reverse transcription-pelymerasechain reaction ( RT-PCR ) assay. Conclusion Sex chromosome lose is the most frequent extral abnormity of t (8 ; 21 ) AML,and is predominantly associated with M2 subtype of AML with unique morphological,immunological and clinical features.
出处
《中国综合临床》
北大核心
2006年第3期221-223,共3页
Clinical Medicine of China