摘要
针对现行培养菌类试验中的菌落计数是采用人工方式完成,在试验样品量较大时,很难快速准确的得到结果的问题,研究开发了一套菌落计数系统。该系统根据RGB色度学原理,利用图像处理技术,可以对培养皿里的白色菌落的数量进行自动检测。在对菌落的色度值分布进行试验研究的基础上,确定了获取分割图像最佳阈值的方法。实验证明此方法不仅计数结果准确,重现性好,而且速度快,计数结果不受菌落接种方法、菌落形态、大小的影响,是实现菌落计数的一种行之有效的方法。
Colony counting in many colony experiments is detected by manual method at present, therefore it is difficult for man to execute the method quickly and accurately .A new automatic colony counting system was developed. Making use of image-processing technology, a study was made on the feasibility of distinguishing objectively white bacterial colonies from clear plates according to the RGB color theory. An optimal chromatic value was obtained based upon a lot of experiments on the distribution of the chromatic value. It has been proved that the method greatly improves the accuracy and efficiency of the colony counting and the counting result is not affected by using inoculation, shape or size of the colony. It is revealed that automatic detection of colony quantity using image-processing technology could be an effective way.
出处
《中国乳品工业》
CAS
北大核心
2006年第2期40-42,共3页
China Dairy Industry
关键词
菌落计数
图像处理
最佳阈值
自动检测
colony counting
image-processing
optimal chromatic value
automatic detection
