摘要
采用80℃热水提取,得到当归水溶性多糖W-ASP。经阴离子交换柱层析、凝胶过滤柱层析对其进行纯化分级。实验结果表明:W-ASP11主要由葡萄糖组成,相对分子质量约为380 000;W-ASP12主要由半乳糖和阿拉伯糖组成,相对分子质量约为19 000;W-ASP2和W-ASP3主要含半乳糖、阿拉伯糖和鼠李糖以及少量葡萄糖和甘露糖,并含有较高的糖醛酸,由单糖组成及红外图谱初步分析,可判断是一种果胶类多糖。当归多糖主要组分W-ASP3经凝胶色谱(GPC)鉴定为均一组分,其相对分子质量约为62 000。
Polysaccharides were fractionated by from Angelica sinensis were extracted with hot water at 80 ℃. They aion-exchange chromatography and gel filtration chromatography respectively. The results showed that W-ASP11 was composed of glucose, with a molecular weight of 380 000; W-ASP12 was composed of galactose and arabinose, with a molecular weight of 19 000; W-ASP2 and W-ASP3 were composed of galactose, arabinose, rhamnose and trace of glucose and mannose, as well as high amount of uronic acid. The substances were considered as pectic polysaccharides from monosaccharide composition and IR spectra. The major fraction W- ASP3 was eluted with a single peak, and the molecular weight of it was determined by HPGPC as 62,000.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2006年第1期1-4,共4页
Journal of Food Science and Biotechnology
基金
国家863计划项目(2002AA248031)
关键词
当归
多糖
分级纯化
结构分析
Angelica .sinensis
polysaccharides
purification
structure analysis