白花蛇舌草对肿瘤细胞信号传导通路的抑制

The Inhibition of Hedyotis diffusa to the Signal Transduction Pathway of Tumor Cells

研究了白花蛇舌草(Hedyotis diffusa)提取物对肿瘤细胞信号传导通路的影响。首先采取四甲基偶氮唑盐微量酶反应比色法(MTT)法测定各提取物对体外培养人宫颈癌细胞株HeLa增殖的抑制;进一步通过免疫印迹法分析了各提取物对EGF介导的Erk信号传导通路中Erk1/2蛋白磷酸化的影响。结果显示,煎煮及冷水提取物对HeLa细胞的增殖均有抑制,但两者无显著差别,而乙醇提取物显著抑制HeLa细胞增殖。对EGF介导Erk信号传导通路的影响显示,白花蛇舌草提取物处理细胞90 min后,EGF诱导的Erk1/2磷酸化水平显著降低,且表现浓度依赖性。抑制效果:乙醇提取物>冷水提取物>煎煮提取物。研究证明,中药白花蛇舌草含有信号传导通路抑制剂,可能在抗肿瘤过程中发挥重要作用。

In order to study the effects of various extracts from the herb Hedyotis diffusa on signal transduction pathway in tumor cells, the inhibition of different extracts to the proliferation of cervix cancer cell line HeLa was measured by MTT. Western blots were used to detect the effects of different extracts on the EGF induced phosphorytation of Erk1/2. The results indicated that decocted- and cold：water-extracts show similar inhibition to HeLa proliferation, and ethanol extracts inhibit proliferation significantly. After 90rain treatment, all of 3 extracts inhibited EGF-induced Erk1/2 phosphoralytion significantly, and inhibition showed dose-dependent manner. Inhibitory affect： ethanol extracts 〉 cold-water-extracts 〉 decocted extracts. The experimental results demonstrated that Hedyotis diffusa contains the signal transduction inhibitor, which may play a important role in the anti-tumor process.