丙泊酚对脓毒症大鼠肝损伤的保护作用

Protective effect of propofol on liver injury in septic mice

目的研究丙泊酚对脓毒症大鼠细胞因子的影响和细胞因子对肝线粒体呼吸功能的影响及其机制。方法采用盲肠结扎穿孔(CLP)制作脓毒症模型,将大鼠随机分为假手术组(C组)、盐水组(NS组)和丙泊酚组(P组),模型制作12h后NS组和P组分别给予生理盐水丙泊酚。在给药前和给药4 h后分别取静脉血,并在给药4h后取肝制备肝线粒体和组织匀浆,分别采用Clark氧电极技术测定线粒体呼吸功能,ELISA法检测IL-1β和IL-6,分光光度法测定NO水平。结果NS组的RCR和ADP/O明显低于C组,丙泊酚能改善其呼吸功能;NS组给药前后IL-1β和IL-6水平明显高于C组,而P组给药4 h后的IL-1β和IL-6水平较NS组明显降低,且NS组NO较C组和P组显著升高。结论丙泊酚能通过降低IL-1β和IL-6水平,减少肝组织NO的产生,从而保护线粒体呼吸功能,改善细胞能量代谢。

Objective To investigate the protective effect of propofol on the liver function in septic mice. Methods Cecal ligation and puncture （CLP） were used to make a sepsis animal model. Healthy mice were randomly divided into control group, NS group,and propofol group （P） with six rats each. The rats of group NS and P were given 0.9% physiological saline or propofol respectively at 12 h after the model establishment. Blood samples were taken before and at 4 h after drugs adminis tration. All mice were sacrificed after 4 h of experiment and the livers were removed for homogenization and mitochondria. The function of mitochodria was evaluated by the clark oxygen eletrode. The level of IL-1β and IL-6 was assayed by ELISA. The level of NO was measured. Results Compared to the control group, the level of RCR, ADP/O decreased significantly and the level of IL-1β, IL-6 increased both before and at 4 h after drugs in NS group. But in group P, the function of mitochondria was improved and the level of IL-I,IL-6, NO was less than that in NS group. Conclusion Propofol can protect the function of mitoehondria through decreasing the level of IL-Iβ, IL-6 and NO in septic mice.